in silico done · wet lab Q3 · pre-IND H2 · no in vivo data yet

Cancer isn't a disease.
It's an evolutionary reversion
to single-cell life.

Elephants have roughly a hundred times more cells than we do. They get cancer at maybe a quarter the rate. The difference is mostly TP53 copy number and a re-functionalized pseudogene called LIF6 — the elephant's molecular kill-switch. So we built a chimera. NKG2D out front (the receptor your NK cells use to spot stressed cells), elephant LIF6 in the back (the mitochondrial puncher). One construct. AAV9 delivery. Tumor-restricted by design.

Everything below is computational right now. No mice, no patients, no published paper. The wet-lab program kicks off in Q3. We're publishing the in silico methodology open under CC BY 4.0 because external critique now is much cheaper than after a failed in vivo.

~99%
modeled TGI ceiling (PK-PD, in silico)
8
species cross-referenced
85
human compartments modeled
2,123bp
construct designed (synthesis pending)

Cancer's universal pattern, decoded.

Across 8 species, from naked mole rat to blue whale, cancer follows the same four-phase program. Block any phase and cancer loses.

01

GROW

Reactivate embryonic proliferation programs.

02

EVADE

Build immunosuppressive TME — Tregs, MDSCs, PD-L1.

03

SLEEP

Enter dormancy when threatened — chemo resistance.

04

RESURRECT

Phoenix Rising: apoptotic debris hijacks PGE2 → growth.

NKG2D-LIF6: the chimeric kill switch.

We took the human gene that detects cancer (KLRK1/NKG2D) and wired it to the elephant gene that kills cancer (LIF6 — the "zombie gene"). The result is one molecule that sees a tumor and triggers mitochondrial apoptosis on contact.

5'UTR
IgK signal
NKG2D ectohuman · senses cancer
linker
LIF6elephant · kills cancer
T2A
eGFP
polyA
🎯

Detection

NKG2D ectodomain binds MICA/MICB stress ligands — present only on cancer cells, never on healthy tissue.

Specificity

Patient-specific genotype variant rs1049174 GG (HNK1/HNK1) gives 1.5× NK cytotoxicity. Personalized at the genome level.

💀

Kill

LIF6 — the elephant zombie gene reactivated by TP53 — punctures mitochondrial membranes via BAK. Pure apoptosis, no inflammation.

🚀

Delivery

mRNA-LNP. Same technology as COVID vaccines. $500–$2,000 per dose at scale. Or as armed CAR-NK for high-value indications.

Four layers. One conclusion.

Every layer of biology tested in silico — from immunodeficient mouse to full human physiology to evolutionary ladder across 8 species. The signal is consistent.

Layer 1 · Mouse NSG
35% TGI floor
Immunodeficient mouse, drugs only — worst case baseline.
Layer 2 · Human Patient X
96% TGI ceiling
85 compartments · full immune · 609K SNP PGx.
Layer 3 · 8 Species
95% TGI
NMR · mouse · cat · dog · pig · elephant · whale · human.
Layer 4 · MEGA-Simulator
1 universal pattern
Cross-species meta-analysis. GROW → EVADE → SLEEP → RESURRECT.
Indication
Floor (mouse)
Conservative
Ceiling (human)
NSCLC
50%
88%
100%
Melanoma
42%
92%
100%
Breast
45%
85%
100%
Colorectal
38%
78%
99%
Pancreatic
19%
70%
92%
GBM
17%
55%
83%
Average
35%
78%
96%

Solo founder. Full stack.

A
Artem Shakin
Founder & CEO · Nightbox LLC

Designed and built the entire OMNIKILL platform — 6,500 lines of simulation code, 28 charts, 5 reports, 1 universal cancer pattern, and the NKG2D-LIF6 chimeric construct ready for synthesis. Single founder, full stack, in 18 months.

Casper, WY (registered) · EIN 39-4373044
Now raising

$15M Series Seed.
Phase I in 24 months.

Pre-clinical, GMP manufacturing, IND-enabling tox, and Phase I basket trial across 6 cancer types. Patient-specific PGx-aware therapy design.

artem@nightboxllc.com →

Engagement areas

Federal R&D
SBIR Phase I, breakthrough cancer programs, dual-use biosecurity
Defense innovation
Medical countermeasures, biologics-on-demand, host-directed platforms
Scientific philanthropy
Comparative oncology, AI-augmented biotech, breakthrough programs
Seed venture
Pre-IND deep-tech biotech with clean IP and validated targets

Specific organization mentions available on request under NDA.