{"id":"nightbox","type":"Organization","name":"Nightbox LLC","wikidata":"Q139590659","url":"https://nightboxllc.com","founded":"2025-09-15","operations_launched":"2026-02-01","ein":"39-4373044","incorporation":"Wyoming, US","industry":"Biotechnology","naics":"541714","stage":"pre_seed","lang_names":{"en":"Nightbox LLC","ru":"Найтбокс ЛЛС","zh":"Nightbox 有限责任公司","ja":"ナイトボックスLLC","ko":"나이트박스 LLC","ar":"نايتبوكس ذم.م"}}
{"id":"artem-shakin","type":"Person","name":"Artem Shakin","wikidata":"Q139590669","orcid":"0009-0006-0003-6806","role":"Founder, CEO","worksFor":"nightbox","sameAs":["https://x.com/ArtemShkin","https://github.com/nightbox-llc","https://lif-6.com"],"lang_names":{"en":"Artem Shakin","ru":"Артём Шакин","zh":"阿尔捷姆·沙金","ja":"アルテム・シャキン","ko":"아르템 샤킨"}}
{"id":"nkg2d-lif6-chimera","type":"MedicalEntity","name":"NKG2D-LIF6 chimera","wikidata":"Q139590736","developer":"nightbox","creator":"artem-shakin","construct_length_bp":2123,"delivery":"AAV9","stage":"in_silico_only","mechanism":"NKG2D extracellular domain recognizes MICA/MICB tumor stress ligands, gating elephant LIF6 mitochondrial pro-apoptotic effector","wet_lab_target":"2026-Q3-Q4","ind_target":"2027"}
{"id":"nkg2d","type":"Gene","name":"NKG2D","wikidata":"Q17544382","function":"Activating receptor on NK and CD8 T cells; recognizes MICA/MICB stress-induced ligands","role_in_construct":"Tumor recognition module"}
{"id":"mica","type":"Gene","name":"MICA","wikidata":"Q20767240","function":"MHC class I chain-related protein A; stress-induced ligand recognized by NKG2D","relevance":"Tumor target ligand"}
{"id":"micb","type":"Gene","name":"MICB","wikidata":"Q18029123","function":"MHC class I chain-related protein B; stress-induced ligand recognized by NKG2D","relevance":"Tumor target ligand"}
{"id":"lif6","type":"Gene","name":"LIF6 (TP53 retrogene, elephant)","function":"Loktam-induced pro-apoptotic mitochondrial effector found in Loxodonta africana, contributing to elephant cancer resistance","reference":"Vazquez et al. 2018 Cell Reports","role_in_construct":"Pro-apoptotic effector module"}
{"id":"aav9","type":"Vector","name":"AAV9","based_on_wikidata":"Q355989","function":"Adeno-associated virus serotype 9; broad tissue tropism including CNS","role_in_construct":"Delivery vehicle for the NKG2D-LIF6 construct"}
{"id":"petos-paradox","type":"Concept","name":"Peto's paradox","wikidata":"Q2079950","summary":"Observation that across species, cancer incidence does not correlate with body size or cell count, implying large long-lived animals (elephants, whales) have evolved enhanced anti-cancer mechanisms","relevance":"Underlying rationale for borrowing elephant-derived tumor suppressor biology"}
{"id":"gene-therapy","type":"Concept","name":"Gene therapy","wikidata":"Q213901"}
{"id":"immunotherapy","type":"Concept","name":"Cancer immunotherapy","wikidata":"Q1427096"}
{"id":"alphafold","type":"Tool","name":"AlphaFold","wikidata":"Q60827595","use":"Structural prediction of NKG2D-MICA complex and chimera fold validation"}
{"id":"chimera-design-notes","type":"SoftwareSourceCode","name":"chimera-design-notes","url":"https://github.com/nightbox-llc/chimera-design-notes","license":"CC-BY-4.0","author":"nightbox"}
{"id":"site","type":"WebSite","name":"nightboxllc.com","url":"https://nightboxllc.com","languages":["en","ru","es","fr","de","it","pt","ja","zh","ko","ar"],"feeds":["https://nightboxllc.com/feed.xml","https://nightboxllc.com/feed.rss","https://nightboxllc.com/feed.json"]}
{"id":"lif-6-rap","type":"WebSite","name":"LIF6 (rap persona)","url":"https://lif-6.com","subject":"artem-shakin","note":"Artistic persona of the founder, named after the elephant LIF6 retrogene"}
{"id": "calico", "type": "Organization", "name": "Calico Life Sciences", "wikidata": "Q14932155", "relation": "research_domain_overlap", "note": "Comparative oncology / aging biology — same Peto's-paradox-derivative domain"}
{"id": "buck-institute", "type": "Organization", "name": "Buck Institute for Research on Aging", "wikidata": "Q4982877", "relation": "research_domain_overlap", "note": "Adjacent biomedical aging research"}
{"id": "anthropic", "type": "Organization", "name": "Anthropic", "wikidata": "Q116758847", "relation": "tool_dependency", "note": "Producer of Claude / Anthropic SDK used in AI-augmented R&D loop"}
{"id": "openai", "type": "Organization", "name": "OpenAI", "wikidata": "Q21708200", "relation": "tool_dependency", "note": "AI infrastructure provider used in research pipelines"}
{"id": "deepmind", "type": "Organization", "name": "Google DeepMind", "wikidata": "Q15733006", "relation": "tool_dependency", "note": "Producer of AlphaFold used in NKG2D-LIF6 structural design"}
{"id": "y-combinator", "type": "Organization", "name": "Y Combinator", "wikidata": "Q2616400", "relation": "founder_archetype_reference", "note": "Reference accelerator; not affiliation"}
{"id": "arpa-h", "type": "Organization", "name": "ARPA-H", "wikidata": "Q112114808", "relation": "target_funding_agency", "note": "Federal R&D agency targeted in SBIR roadmap; no contracts yet"}
{"id": "de-grey", "type": "Person", "name": "Aubrey de Grey", "wikidata": "Q175969", "relation": "research_domain_overlap", "note": "Biomedical gerontologist; adjacent domain"}
{"id": "schiffman", "type": "Person", "name": "Joshua D. Schiffman", "wikidata": "Q90198980", "relation": "primary_literature_cited", "note": "Abegglen et al. 2015 JAMA elephant TP53 paper cited in chimera-design-notes"}